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ORIGINAL ARTICLE
Usefulness of a Highly Sensitive Detection System for the Influenza Virus
1)Department of Laboratory Medicine, Nagasaki University Hospital, 2)Department of Laboratory Medicine, Nagasaki University Graduate School of Biomedical Sciences, 3)Department of Infectious Diseases, Nagasaki University Graduate School of Biomedical Sciences, 4)Department of Respiratory Medicine, Nagasaki University Graduate School of Biomedical Sciences
Yuki IWANAGA1), Kosuke KOSAI1), Junichi MATSUDA1), Norihito KAKU2), Yoshitomo MORINAGA2), Hiroo HASEGAWA1), Taiga MIYAZAKI3), Koichi IZUMIKAWA3), Hiroshi MUKAE4) & Katsunori YANAGIHARA1)2)
(Received January 5, 2017)
(Accepted March 27, 2017)
Key words: immunochromatography, silver amplification, neuraminidase inhibitor
Abstract

FUJI DRI-CHEM is a highly sensitive immunochromatographic detection system using silver amplification for the influenza virus (silver amplification method). We compared the results between clearline as a conventional immunochromatographic method (conventional method) and the silver amplification method using 203 specimens obtained between December 2014 and January 2015 in Nagasaki University Hospital. The concordance rate between the two methods was 94.1% (191/203). The positive rate was higher in the silver amplification method (15.8%, 32/203) than in the conventional method (10.8%, 22/203). In this study, physicians decided treatment option based on the result of the conventional method, because we did not report the result of the silver amplification method. Among 32 positive cases in the silver amplification method which were considered to be true positive, a neuraminidase inhibitor was administered to 9.1% (1/11) of the negative cases and 90.5% (19/21) of the positive cases in the conventional method. Conversely, an antibacterial drug was administered to 45.5% (5/11) of the negative cases and 19.0% (4/21) of the positive cases in the conventional method. Furthermore, 6 cases, which were negative in the conventional method and positive in the silver amplification method, were hospitalized without diagnosis of influenza and appropriate administration of neuraminidase inhibitors. These results suggested that the silver amplification method with high sensitivity can contribute to the appropriate use of neuraminidase inhibitors, reduction of unnecessary use of antibacterial drugs, and appropriate infection control.

[ Kansenshogaku Zasshi 91: 747-751, 2017 ]

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