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The Relationship Between Toxin Production and POT Type in Methicillin-resistant Staphylococcus aureus Clinical Isolates Producing an Exfoliative Toxin or Panton-Valentine Leukocidin
1)Department of Clinical Laboratory, Ehime University Hospital, 2)Ishizuchi Eye Clinic, 3)Department of Pediatrics, Ehime University Hospital, 4)First Department of Internal Medicine, Ehime University Hospital
Hitoshi MIYAMOTO1), Takashi SUZUKI2), Shinobu MURAKAMI1), Fumihiro OCHI3), Koichiro SUEMORI4) & Hisamichi TAUCHI3)
(Received February 6, 2018)
(Accepted May 10, 2018)
Key words: methicillin-resistant Staphylococcus aureus (MRSA), exfoliative toxin (ET), Panton-Valentine Leukocidin (PVL), PCR-based open reading frame typing (POT)

Although an exfoliative toxin (ET) or Panton-Valentine Leukocidin (PVL) may play a critical role in the pathogenesis of methicillin-resistant Staphylococcus aureus (MRSA) infection, little is known about genetic diversity among MRSA isolates producing ET or PVL. In this study, we investigated the PCR-based open reading frame typing (POT) along with toxin type and drug susceptibility in 40 strains of ET or PVL-producing MRSA isolated at Ehime University Hospital from January 2010 to December 2017.

Since all 22 ET-A and 10 ET-B producing strains showed 70-18-81 and 73-152-80, respectively, ET producing strains could be the same clones. A drug susceptibility test revealed that the ET-A strain and the ET-B strain showed the same pattern of 80% and 100%, respectively. Three kinds of PVL-producing strains were shown as follows, 106-77-113 (5 strains), 64-88-19 (2 strains), and 110-16-49 (1 strain). Thus it was likely that they were multiple clones.

The POT typing is useful to detect ET producing strains because they could be the same clone in the POT typing. Although multiple clones were detected in the PVL strain, the strains with 106-77-113 and POT1 type of 64, 110 might be PVL producing strains. Since the classification of POT correlates with ET and PVL toxin production, it could be an effective method to identify toxin strains.

[ Kansenshogaku Zasshi 92: 663-669, 2018 ]

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